This chapter introduces how to differentiate instruction in an online environment. Fostering successful online learning communities to meet the diverse needs of students is a challenging task. Since the “one size fits all” approach is not realistic in a face-to-face or online setting, it is essential as an instructor to take time to understand differentiation and to work in creating an online learning environment that responds to the diverse needs of learners. It is our responsibility to ensure that the teaching and learning that takes place online is not only accessible, but of quality. The author hopes that developing an understanding of differentiation and specific instructional strategies to differentiate online will inform the learner of ways to maximize learning by addressing the diverse needs of students.