Virtual teamwork in the e-learning classroom has provided opportunities for merging social theory and learning theory, mixing technology, culture, identity, and community. Online learning teams have generated attention to the social and cultural characteristics that influence these global interactions. This chapter discusses the prevalence of eight traditional dimensions of culture occurring during online learning team interaction. A study with graduate students, who were experienced in virtual teamwork, provides quotes and examples of experiences, challenges, and suggestions for improvement to the multi-cultural, virtual team experience. The students’ suggestions inform guidelines for e-learning faculty and students, while additional study results present understanding of the acculturation process, a process that occurs when diversified social and cultural characteristics come together and form a cultural hybrid to accomplish e-learning team goals.