Looking Into the Binary Interactome of Enterobacteriaceae Family of Bacteria

Looking Into the Binary Interactome of Enterobacteriaceae Family of Bacteria

Saritha Namboodiri (University of Calicut, Malappuram, India) and Alessandro Giuliani (Istituto Superiore di Sanità, Roma, Italy)
Copyright: © 2019 |Pages: 16
DOI: 10.4018/IJARB.2019010104


Protein-protein interactions (PPIs) regulate most of the biological activities within a cell. A set of pairwise PPIs in seven genera of bacterial pathogens (Salmonella, Escherichia, Shigella, Yersinia, Klebsiella, Photorhabdus, and Pantoea) of Enterobacteriaceae family was analysed. At genotypic level, the correlation coefficient analysis of the mutation spectra of the ten sets of directly interacting protein partners in Escherichia coli recognised all the ‘interacting partners' in Escherichia coli. Extending the correlation analysis to include strains from the rest of the bacterial genera decreased the recognition efficiency providing quantitative evidence that binary interactome have incomplete superposition across species. At phenotype level, a reliable classification of bacterial pathogens was obtained by measuring PPI variations in terms of between phylogenetic distance correlation distances among ten sets of proteins partners. This forces us to rethink upon the possibility of PPI rewiring with a consequent change in physiological role of the same protein.
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At present, protein–protein interaction studies focus on the combinatorial complexity of functional interactome with an intention to build a viable cell from them. The bewildering combinatorial complexity of possible interactomes raises many questions on development as well as rewiring of PPIs along evolution. Peter Tompa and George Rose sketched a minimalistic combinatorial model (IJARB.2019010104.m01possible protein-protein interaction patterns) for yeast and concluded that a prior framework of existing interactions is essential, as emergent interactome cannot self-organise spontaneously (Tompa & Rose, 2011). In evolutionary terms, this implies that a particular PPI pattern (an interactome) is highly conserved physical entity and small variations are important phenotypic markers of species separation (Tompa & Rose, 2011; Kyaw, Pawan, Maria & Alessandro. 2006; Goh, Bogan, Joachimiak, Walther & Cohen, 2000). The analysis of eventual variation in the PPI patterns from the orchestrated protein interactions is thus of crucial importance in taxonomy.

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