Quantitative Structure–Activity Relationship Studies of Anticancer Activity for Isatin (1H-indole-2,3-dione) Derivatives Based on Density Functional Theory

Quantitative Structure–Activity Relationship Studies of Anticancer Activity for Isatin (1H-indole-2,3-dione) Derivatives Based on Density Functional Theory

Samir Chtita, Mounir Ghamali, Majdouline Larif, Rachid Hmamouchi, Mohammed Bouachrine, Tahar Lakhlifi
DOI: 10.4018/IJQSPR.2017070108
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To establish a QSAR of anticancer activity for Isatin derivatives, a series of Isatin derivatives were analyzed by principal component analysis, multiple linear regression, partial least squares and multiple nonlinear regression analysis. The authors proposed linear and nonlinear models and interpreted the activity of the compounds by multivariate statistical analysis. The proposed models were used to predict the activity of test set compounds, and an agreement between experimental and predicted values was verified. The applicability domain of MLR models was investigated using William's plot to detect outliers and outsides compounds. For the successful application of the developed models to predict new compounds, rigorous validation tests have been used in this direction. Additionally, the rm2 metrics have been used to ensure the close agreement of predicted response data with observed ones. The developed models have been used for designing some new Isatin derivatives with high predicted values of anticancer effect.
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Isatin (1H-indole-2,3-dione) is an indole derivative and an important class of heterocyclic compounds found in many plants, such as Isatistinctoria, Calanthe discolor and Couroupitaguianensis (Da Silva, Garden & Pinto, 2001). It is also found in humans as a metabolic derivative of adrenaline (Sonawane & Tripathi, 2013).

Isatin was first obtained as a product from chromic acid oxidation of indigo dye by Erdmann and Laurent (Erdmann, 1840; Laurent, 1840) in 1841, and their synthetic derivatives are important substrates used for the synthesis of a variety of heterocyclic compounds, and used as raw materials for drug synthesis. Isatin and its derivatives are well known therapeutic agents due to their wide range of pharmacological and biological activities including anticancer (Lee, Long, Murray et al., 2001; Chapman, Magee, Stukenbrok et al., 2002), anticonvulsant (Verma, Pandeya, Singh et al., 2004), antiviral (Sriram Tanushree & Yogeeswari, 2004; Pirrung, Pansare, Sarma et al., 2005), antibacterial and antifungal (Chohan, Pervez, Rauf et al., 2004), anti-HIV and anti-inflammatory activities (Lashgari & Ziarani, 2011; Mishra & Bauerle, 2012; Walker, Kim & Nguyen, 2012). Isatinis used as a starting point in the synthesis of oligomeric or polymeric structures which are used in the field of solar energy (Zhang, Fu, Xie et al., 2011), organic memory devices creation (Xu Li, Liu et al., 2011) and organic field effect transistors (Lei, Cao, Fan et al., 2011; Ashraf, Kronemeijer, James et al., 2012).

Many methodologies have been adopted to synthesize Isatin derivatives and to explore their possible role in the treatment of various diseases. Among these protocols, the method developed by Sand-Meyer is the oldest and the most frequently used for the synthesis of Isatin (Rehn, 2004). This method involves the reaction of aniline with chloral hydrate and hydroxylamine hydrochloride in aqueous sodium sulfate to form an isonitrosoacetanilide, which after isolation, when treated with concentrated sulfuric acid produces Isatin.

Looking at the literature, it can be seen that in particular, halogenated isatin derivatives have been reported to exhibit anticancer activity. 5-Bromo-3-o-nitrophenyl isatinhydrazone and 5-bromo-(2-oxo-3-indolinyl) thiazolidine-2,4-diones substituted by various Mannich bases were found to exhibit anticancer activity against Walker carcinoma-256 and P388 lymphocytic leukemia in mice, respectively (Esheba & Salama, 1985; Popp & Pajouhesh, 1983). 6-Bromo-2- methylthio-3H-indol-3-one, tyrindoleninone, a brominated precursor to Tyrian purple, isolated from the egg masses of the Australian mollusk Dicathaisorbita has been reported as a cytotoxic marine compound (Westley, Vine, Benkendorff et al., 2006). 6-bromoisatin, a major decomposition product formed through the oxidation of tyriverdin (precursor of Tyrian purple), has been shown to have a weaker anti-cancer activity against a human lymphoma cell line in comparison with 6-Bromo-2- methylthio-3H-indol-3-one (Westley, Vine, Benkendorff et al., 2006; Benkendorff, Bremner & Davis, 2001). 5,7-Dibromoisatin,a significantly more potent as a cytotoxin than Isatin against U937 (human monocyte-like histiocytic lymphoma) cells (Vine, Locke, Ranson et al., 2007), its N-benzyl derivatives with more cytotoxicity toward these lymphoma cells and activity against a range of human cancer cell lines including a metastatic breast adenocarcinoma cell line (MDA-MB-231) (Vine, Locke, Ranson et al., 2007), and cytotoxic N-alkylhaloisatins are some examples of reported anticancer halogenated Isatins in recent researches (Vine, Locke, Ranson et al., 2007; Matesic, Locke, Bremner et al., 2008). In the recently approved drugs by FDA, a 5-fluoro-3-substituted-2-oxoindole, SU11248 (Sutent) is provided for the treatment of gastrointestinal stromal tumors and advanced renal-cell carcinoma (Prenen, Cools, Mentens et al., 2006; Motzer, Michaelson, Redman et al., 2006).

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