Green Synthesis of Calcium Carbonate-Zinc Oxide Nanocomposites (NPs) Methodology, Characterization, and Antimicrobial Property Evaluation: Synthesis, Characterization, and Antimicrobial Evaluations

Abstract

This chapter presents an innovative approach to synthesizing calcium carbonate-zinc oxide (CaCO3-ZnO) nanocomposites using a green synthesis method. Utilizing silk fibroin wash water, this environmentally friendly technique enables the formation of stable nanocomposites without harmful chemicals. Detailed characterization confirmed the successful synthesis, composition, and structural properties of the CaCO3-ZnO nanocomposites. The antimicrobial activity tests demonstrated significant antibacterial activity against common pathogens. In the MTT assay, green synthesized NPs were administered at varying concentrations, showing less than 50% inhibition, indicating no significant cytotoxicity. The findings highlight the potential of CaCO3-ZnO nanocomposites for biomedical and dental applications, offering a sustainable alternative for antimicrobial agents. This chapter underscores the importance of green synthesis in advancing nanotechnology and provides insights into the future applications of nanocomposites in healthcare.