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What is 18S rRNA

Analysis, Conservation, and Restoration of Tangible and Intangible Cultural Heritage
Ribosomal RNA of the 18S sub-unit, is an RNA sequence that is transcribed from a gene within the nucleolar region of the cell. Once transcribed, the RNA binds proteins to be part of the small subunit of the ribosome called 18S.
Published in Chapter:
Molecular Identification of Fungi in Outdoor Sandstones of Cultural Heritage Buildings for Modeling Their Biodeterioration
José Luis González Montoya (National Autonomous University of Mexico, Mexico), José de Jesús Pérez Bueno (Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Mexico), Maria del Carmen Cano Canchola (Guanajuato University, Mexico), Jorge A. Cervantes-Jáuregui (Guanajuato University, Mexico), Veridiana Reyes (Guanajuato University, Mexico), and Aurelio Álvarez-Vargas (Guanajuato University, Mexico)
DOI: 10.4018/978-1-5225-6936-7.ch007
Abstract
Our cultural heritage kept in sandstone is doubtless suffering from accelerated biodeterioration. Fungi play a key role, either by acid production, dye secretion or as microbiont in the symbiotic association of the lichen with algae. The use of the universal initiators for the amplification of conserved regions of the ribosomal genes has been serving as an excellent marker for the microorganism identification, due to the ubiquity and conservation of these regions present in the genome of the eucarionts. In this chapter, the different fungi species present in sandstone were identified using molecular analysis techniques, as amplifiers in genes chains and the sequentiation of the resultant fragments. A theoric model of the way that the fungus contributes to the biodeterioration of monuments is proposed, with the objective of a better understanding of the contributions of these microorganisms in the general process of biodeterioration.
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