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What is Polymerase Chain Reaction (PCR)

Tactical Sciences for Biosecurity in Animal and Plant Systems
A molecular test method utilizing an enzyme to rapidly make copies of DNA or RNA, usually coupled with a mechanism to specifically target and identify the nucleic acid sequence of a known pathogen.
Published in Chapter:
Training, Tests, and Tech: Deployment of Diagnostic Tools for Biosecurity
Carrie Lapaire Harmon (University of Florida, USA), Bruce L. Akey (Texas A&M Veterinary Medical Diagnostic Laboratory, USA), Francisco Manuel Ochoa-Corona (Oklahoma State University, USA), Akhilesh Ramachandran (Oklahoma State University, USA), and Poonam Sharma (Oklahoma State University, USA)
Copyright: © 2022 |Pages: 40
DOI: 10.4018/978-1-7998-7935-0.ch007
Abstract
Diagnosis of disease is a process of hypothesis, investigation, and synthesis. Regardless of whether a human, animal, or plant is afflicted, the process of diagnosis is strikingly similar. Positioned on the biosecurity continuum between surveillance and response, early and accurate diagnosis is critical to effective mitigation and management of disease. Infectious diseases have the potential to spread among animal or plant populations, jump species barriers, and result in epidemics and global pandemics. Additionally, zoonotic infectious agents can also significantly impact human health on a mass scale. It is critical that infectious diseases be identified and detected in a timely fashion to prevent spread. This chapter will delve into the resources and supporting activities for that process, demonstrated via case studies from animal and plant systems, illuminating similarities and differences in the diagnostic process tools that can be mobilized and enhanced for biosecurity.
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Recent Advances on Nanomaterials for Diagnostic, Treatment, and Prevention of COVID-19
A technique for rapidly making millions to billions of copies of a DNA sample, allowing it to be enlarged for more detailed analysis.
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Non-Tsetse-Transmitted Animal Trypanosomosis (NTT): Trypanosomosis Due to Trypanosoma evansi (Surra), T. vivax (Duttonella), and T. equiperdum
Is a technique used to make numerous copies of a specific segment of DNA quickly and accurately to enable investigators obtain large quantities of DNA that are required for various experiments and procedures in molecular biology, forensic analysis, evolutionary biology and biomedical diagnosistics.
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Biotechnology: Recent Developments, Emerging Trends, and Implications for Business
A method widely used to rapidly make millions to billions of copies of a specific DNA sample through thermal cycling with primers and DNA polymerase.
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Viromechanics of Fluid: A Novel Computational Method to Model the COVID-19 Transmission
Polymerase chain reaction (PCR) is a process that amplifies (replicates) a small, well-defined segment of DNA many hundreds of thousands of times, creating enough of it for analysis. Test samples are treated with certain chemicals that allow DNA to be extracted. Reverse transcription converts RNA into DNA. Reverse transcription-polymerase chain reaction (RT-PCR) first uses reverse transcription to obtain DNA, followed by PCR to amplify that DNA, creating enough to be analyzed. RT-PCR can thereby detect SARS-CoV-2, which contains the only RNA. The RT-PCR process generally requires a few hours. These tests are also referred to as molecular or genetic assays.
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Genes: Recombinant DNA Technology
A technique used to make copies of a target piece of DNA.
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Legal Review of the Government's Responsibility in Compensating for the Damage Caused by the COVID-19 Crisis
Polymerase chain reaction (PCR) is a process that amplifies (replicates) a small, well-defined segment of DNA many hundreds of thousands of times, creating enough of it for analysis. Test samples are treated with certain chemicals that allow DNA to be extracted. Reverse transcription converts RNA into DNA. Reverse transcription-polymerase chain reaction (RT-PCR) first uses reverse transcription to obtain DNA, followed by PCR to amplify that DNA, creating enough to be analyzed. RT-PCR can thereby detect SARS-CoV-2, which contains the only RNA. The RT-PCR process generally requires a few hours. These tests are also referred to as molecular or genetic assays.
Full Text Chapter Download: US $37.50 Add to Cart
Extremophiles in Sustainable Bioenergy Production as Microbial Fuel Cells
Method used to make millions to billions copies of specific DNA sequence.
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