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What is VIS Spectrophotometry

Chapter 6
Quality Control Applications in the Pharmaceutical and Medical Device Manufacturing Industry
Refers to absorption spectroscopy in the visible spectral region; is used to determine the absorption or transmission of VIS light (400 to 820 nm) by a sample. It can also be used to measure concentrations of absorbing materials based on developed calibration curves of the material.
Published in Chapter:
Multi-Wavelength Spectrophotometric Analysis: Determination of Sitagliptin and Metformin in Tablets
Eugenia Gabriela Carrillo-Cedillo (Universidad Autónoma de Baja California, Mexico), Maria del Pilar Haro-Vazquez (Universidad Autónoma de Baja California, Mexico), Nataly Gómez-Carrillo (Universidad Autónoma de Baja California, Mexico), Ruben Guillermo Sepulveda Marques (Universidad Autónoma de Baja California, Mexico), and Mónica Graciela Coronel (Universidad Nacional de Santiago del Estero, Argentina)
Copyright: © 2022 |Pages: 23
DOI: 10.4018/978-1-7998-9613-5.ch006
Abstract
On the basis of the absorbance activity law, it is possible to carry out the spectrophotometric determination of two individual components (having totally or partially overlapping spectra) in a binary mixture. This determination can be carried out in a very precise and simple way by introducing ourselves in the multicomponent analysis by means of the so-called multi-wavelength linear regression analysis (MLRA) method that allows easy handling of data obtained at different wavelengths and whose results are comparable in accuracy and precision with other mathematical procedures. Another way of being able to calculate the individual concentration of two components is a mixture when their spectra are totally or partially overlapping; it is the derived spectrophotometry. The objective of this work is to apply the MLRA method for the individual determination of a binary mixture of metformin and sitagliptin as well as to apply derived spectrophotometry.
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Chapter 8
Optimization of a Spectrophotometric Flow Injection: Method for Determination Copper and Manganese in Wines by Design Experiments
Refers to absorption spectroscopy in the visible spectral region; is used to determine the absorption or transmission of VIS light (400 to 820 nm) by a sample. It can also be used to measure concentrations of absorbing materials based on developed calibration curves of the material.
Published in Chapter: Optimization of a Spectrophotometric Flow Injection: Method for Determination Copper and Manganese in Wines by Design Experiments; From: Design of Experiments for Chemical, Pharmaceutical, Food, and Industrial Applications
Full Text Chapter Download: US $37.50 Add to Cart
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