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Dynamical Analysis of Drug Efficacy and Mechanism of Action Using GFP Reporters

Dynamical Analysis of Drug Efficacy and Mechanism of Action Using GFP Reporters

Jianping Hua, Chao Sima, Milana Cypert, Edward R. Dougherty, Jeffery M. Trent, Michael L. Bittner
Copyright: © 2016 |Pages: 38
ISBN13: 9781522503538|ISBN10: 1522503536|EISBN13: 9781522503545
DOI: 10.4018/978-1-5225-0353-8.ch009
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MLA

Hua, Jianping, et al. "Dynamical Analysis of Drug Efficacy and Mechanism of Action Using GFP Reporters." Emerging Research in the Analysis and Modeling of Gene Regulatory Networks, edited by Ivan V. Ivanov, et al., IGI Global, 2016, pp. 316-353. https://doi.org/10.4018/978-1-5225-0353-8.ch009

APA

Hua, J., Sima, C., Cypert, M., Dougherty, E. R., Trent, J. M., & Bittner, M. L. (2016). Dynamical Analysis of Drug Efficacy and Mechanism of Action Using GFP Reporters. In I. Ivanov, X. Qian, & R. Pal (Eds.), Emerging Research in the Analysis and Modeling of Gene Regulatory Networks (pp. 316-353). IGI Global. https://doi.org/10.4018/978-1-5225-0353-8.ch009

Chicago

Hua, Jianping, et al. "Dynamical Analysis of Drug Efficacy and Mechanism of Action Using GFP Reporters." In Emerging Research in the Analysis and Modeling of Gene Regulatory Networks, edited by Ivan V. Ivanov, Xiaoning Qian, and Ranadip Pal, 316-353. Hershey, PA: IGI Global, 2016. https://doi.org/10.4018/978-1-5225-0353-8.ch009

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Abstract

To the development of effective cancer drug, it is necessary to, first, identify drugs and their possible combinations that could exert desired control over the type of cancer being considered; second, have a drug testing method that allows one to assess the variety of responses that can be provoked by drugs. To facilitate such an experiment-modeling-experiment cycle for drug development, a method based on the dynamical systems of pathways is presented. It involves a three-state experimental design: (1) formulate an oncologic pathway model of relevant cancer; (2) perturb the pathways with the drugs of known effects on components of the pathways of interest; and (3) measure process activity indicators at various points on cell populations. To evaluate the drug response in a high-throughput manner, a green fluorescent protein reporter-based technology has been developed. The authors apply the dynamical approach to several issues in the context of colon cancer cell lines.

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