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Correlation and Analysis of Overlapping Leukocytes in Blood Cell Images Using Intracellular Markers and Colocalization Operation

Correlation and Analysis of Overlapping Leukocytes in Blood Cell Images Using Intracellular Markers and Colocalization Operation

Balanagireddy G., Ananthajothi K., Ganesh Babu T. R., Sudha V.
Copyright: © 2021 |Pages: 18
ISBN13: 9781799830924|ISBN10: 1799830926|EISBN13: 9781799830931
DOI: 10.4018/978-1-7998-3092-4.ch008
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MLA

G., Balanagireddy, et al. "Correlation and Analysis of Overlapping Leukocytes in Blood Cell Images Using Intracellular Markers and Colocalization Operation." AI Innovation in Medical Imaging Diagnostics, edited by Kalaivani Anbarasan, IGI Global, 2021, pp. 137-154. https://doi.org/10.4018/978-1-7998-3092-4.ch008

APA

G., B., K., A., T. R., G. B., & V., S. (2021). Correlation and Analysis of Overlapping Leukocytes in Blood Cell Images Using Intracellular Markers and Colocalization Operation. In K. Anbarasan (Ed.), AI Innovation in Medical Imaging Diagnostics (pp. 137-154). IGI Global. https://doi.org/10.4018/978-1-7998-3092-4.ch008

Chicago

G., Balanagireddy, et al. "Correlation and Analysis of Overlapping Leukocytes in Blood Cell Images Using Intracellular Markers and Colocalization Operation." In AI Innovation in Medical Imaging Diagnostics, edited by Kalaivani Anbarasan, 137-154. Hershey, PA: IGI Global, 2021. https://doi.org/10.4018/978-1-7998-3092-4.ch008

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Abstract

This chapter contributes to the study of uncertainty of signal dimensions within a microscopic image of blood sample. Appropriate colocalization indicator classifies the leukocytes in the region of interest having ragged boundaries. Signal transduction has been interpreted using correlation function determined fluorescence intensity in proposed work using just another colocalization plugin (JaCoP). Dependence between the channels in the colocalization region is being analysed in a linear fashion using Pearson correlation coefficient. Manders split, which gives intensity, is represented in a channel by co-localizing pixels. Overlap coefficients are also being analysed to analyse coefficient of each channel. Li's intensity correlation coefficient is being used in specific cases to interpret the impact of staining.

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