When dealing with human lives, the need to utilize and apply the latest technology to help in saving and maintaining patients’ lives is quite important and requires accurate, near-real-time data acquisition and evaluation. At the same time, the delivery of a patient’s medical data needs to be as fast and as secure as possible. One possible way to achieve this is to use a wireless framework based on radio-frequency identification (RFID). This framework can integrate wireless networks for fast data acquisition and transmission while maintaining the privacy issue. This chapter discusses the development of an agent framework in which RFID can be used for patient data collection. The chapter presents a framework for the knowledge acquisition of patient and doctor profiling in a hospital. The acquisition of profile data is assisted by a profiling agent that is responsible for processing the raw data obtained through RFID and a database of doctors and patients.