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What is RT-PCR

Chapter 18
Handbook of Research on Green Synthesis and Applications of Nanomaterials
Real time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus. Originally, the method used radioactive isotope markers to detect targeted genetic materials, but subsequent refining has led to the replacement of isotopic labelling with special markers, most frequently fluorescent dyes. This technique allows scientists to see the results almost immediately while the process is still ongoing, whereas conventional RT–PCR only provides results at the end of the process.
Published in Chapter:
Use of Nanoparticles to Combat COVID-19
Ashish Kumar (Lovely Professional University, India) and Abhinay Thakur (Lovely Professional University, India)
Copyright: © 2022 |Pages: 29
DOI: 10.4018/978-1-7998-8936-6.ch018
Abstract
On January 30, 2020, the World Health Organization declared the unexpected coronavirus pneumonia pandemic a public health crisis of global significance. COVID-19 in its most severe form can cause coagulation issues and acute respiratory distress syndrome. Due to viral modifications and the advent of new viral strains, the efficiency of traditional treatments for viral infections is rapidly fading. To overcome the limitations and to improve anti-viral treatments, integrated scientific research toward nanotechnology treatment investigations are anticipated for probable use in the prevention and/or treatment of viral infections. This chapter focuses on nano-based diagnostics, accompanied by a study of nano-based therapeutics and treatments that have been shown to be effective against viruses that are closely related to SARS-CoV-2. Furthermore, the authors anticipate that nanotechnology-based approaches mentioned throughout this chapter will aid researchers in developing new COVID-19 prevention, diagnosis, and treatment methods.
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Chapter 8
Smart Diagnostics of COVID-19 With Data-Driven Approaches
A technique that can compound transcription of RNA into DNA and apply polymerase chain reaction to increase DNA targets.
Published in Chapter: Smart Diagnostics of COVID-19 With Data-Driven Approaches; From: Applied Big Data Analytics and Its Role in COVID-19 Research
Full Text Chapter Download: US $37.50 Add to Cart
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